Coding

Part:BBa_K5310007:Design

Designed by: Ilias Pilianidis   Group: iGEM24_thessaloniki   (2024-09-29)


CD8a Hinge (homo sapiens codon optimized)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 92
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimised for use in human primary cell lines.


Source

Homo sapiens chromosome 2 (UniProt P01732).

References